CRYSTAL TAQ DNA POLYMERASE

WORLD’S FIRST TAQ DNA POLYMERASE
FREE FROM BACTERIAL & ANIMAL DNA CONTAMINATION!


Following a breakthrough in enzyme production, Pharmozyme is proud to introduce the world’s first Taq polymerase free from background bacterial animal DNA contamination. This first truly pure enzyme will greatly aid new generations of sequencing technologies, while being a superior component to pathogen testing in Clinical Medicine, Food-Borne contamination, Quality Assurance, Forensics, Water Treatment, Veterinary & Agriculture and Bioterrorism.

FEATURES:

  1. Engineered for highly sensitive applications; qPCR and RT-PCR.
  2. High yield and zero contamination, insuring sample longevity
  3. Cleaner Amplicons for Pyrosequencing.

 

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PRODUCT DESCRIPTION

Pharmozyme’s Crystal Taq DNA polymerase is derived from Thermus aquaticus. It is a thermostable enzyme with 5’—–3’ DNA polymerase activity and 5′—–3’ exonuclease activity, while lacking 3’—–5’ exonuclease (proofreading) activity. The enzyme is free of animal & bacterial DNA contamination – eliminating interfering background.

Applications

  • Gene amplification (PCR) & sequencing – Avoiding bacterial DNA contamination minimizes non-specific amplification of target regions. Current contamination levels in supplier products reduce the quality and accuracy of whole genome sequencing. The technology engineered into Crystal Taq greatly increases the efficiency of random genome amplification for sequencing.
  • RT-PCR – Resulting higher sensitivity, increases overall accuracy. Crystal Taq impact reduces repeat testing, saving time & money.
    Pyrosequencing & Sanger sequencing – Crystal Taq creates cleaner amplicons resulting in higher resolution sequencing result.
  • Clinical Diagnostics, Food Pathogen Testing, Forensics, Quality Assurance Testing, Veterinary, Agriculture, Water Treatment and Bioterrorism – Elimination of False positive results caused by DNA contamination provides superior accuracy – saving lives and $Billions of dollars in those industries.

ADDITIONAL INFORMATION

Weight 5 lbs
Dimensions 11.25 x 9.5 x 8 in
Volume 50U, 100U, 250U, 500U


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Packaging size: 5 units / µL
Storage temperature: -15°C to -25°C
Materials supplied:
  • One vial (Variable Size) of Crystal Taq polymerase in Taq storage buffer
  • One vial (1.5 ml) of 10X PCR buffer
  • One vial (1.5 ml) of 25 mM MgCl2
Taqstorage buffer: 20 mM Tris, pH 8.0
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% IGEPAL CA-630
50% glycerol
PCR buffer (10X): 200 mM Tris, pH 8.3 at 4°C
500 mM KCl
15 mM MgCl2

PCR mix:

 50 reaction volume  Final concentration

Water (ddH2O)

 adjust for 50 µl final volume
10X PCR buffer  5 µl  1X
10 mM dNTPs  1 µl  0.2 mM of each
10 µM Forward primer  1.5 µl  0.3 µM
10 µM Reverse primer  1.5 µl  0.3 µM
Template DNA  Varies  < 0.02 µg/µl
Taq DNA polymerase   µl  2.5 U/50 µl



PCR cycling set-up:

Initial Denaturation 95°C for 2 min
Cycling (25-35 cycles)
Denaturation 95°C for 30 sec
Annealing 0-68°C for 30 sec
Extension 72°C for 1 min/kb
Final Extension 72°C for 5 min
Hold 4°C indefinitely

Notes:

DNA templates should preferably be pure. Chemical agents, ionic detergents (such as SDS) and other DNA damaging agents should be avoided. For routine applications, 25 to 35 PCR cycles is usually enough to provide sufficient copies of the target sequence.

Usage:

This product is for research use only in molecular biology applications and is not intended for use in diagnostic procedures. Purchase of this product does not include a license to perform any patented application; therefore it is the users responsibility to determine if any license agreement might be needed prior usage of this product. Practicing real-time require additional licensing from Roche or Applied Biosystems.

References:

  1. Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. (1990). PCR Protocols: A
    Guide to Methods and Applications. Academic Press, New York.
  2. Kellogg, D. E., Rybalkin, I., Chen, S., Mukhamedova, N., Vlasik, T. et al. (1994)
    Biotechniques 16(6):1134-7.
  3. Mullis, K. B. (1991) PCR Methods Appl 1(1):1-4.

  4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  5. D’Aquila, R. T., Bechtel, L. J., Videler, J. A., Eron, J. J., Gorczyca, P. et al. (1991)
    Nucleic Acids Res 19(13):3749.
  6. Chou, Q., Russell, M., Birch, D. E., Raymond, J. and Bloch, W. (1992) Nucleic Acids
    Res 20(7):1717-23.
  7. Erlich, H. A., Gelfand, D. and Sninsky, J. J. (1991) Science 252(5013):1643-51.
    Guide to Methods and Applications. Academic Press, New York.