CRYSTAL TAQ PCR MASTER MIX (2x)

WORLD’S FIRST TAQ DNA POLYMERASE
FREE FROM BACTERIAL & ANIMAL DNA CONTAMINATION!

Designed for the most sensitive applications such as qPCR and RT-PCR, as well as making cleaner amplicons for Pyrosequencing, our first master mix is built to simplify gene amplification.

FEATURES:

  1. Engineered for highly sensitive applications; qPCR and RT-PCR.
  2. High yield and zero contamination, insuring sample longevity
  3. Cleaner Amplicons for Pyrosequencing.

 

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PRODUCT DESCRIPTION

Pharmozyme’s Crystal Taq DNA polymerase is derived from Thermus aquaticus. It is a thermostable enzyme with 5’—–3’ DNA polymerase activity and 5′—–3’ exonuclease activity, while lacking 3’—–5’ exonuclease (proofreading) activity. The enzyme is free of animal & bacterial DNA contamination – eliminating interfering background.

Applications

  • Gene amplification (PCR) & sequencing – Avoiding bacterial DNA contamination minimizes non-specific amplification of target regions. Current contamination levels in supplier products reduce the quality and accuracy of whole genome sequencing. The technology engineered into Crystal Taq greatly increases the efficiency of random genome amplification for sequencing.
  • RT-PCR – Resulting higher sensitivity, increases overall accuracy. Crystal Taq impact reduces repeat testing, saving time & money.
    Pyrosequencing & Sanger sequencing – Crystal Taq creates cleaner amplicons resulting in higher resolution sequencing result.
  • Clinical Diagnostics, Food Pathogen Testing, Forensics, Quality Assurance Testing, Veterinary, Agriculture, Water Treatment and Bioterrorism – Elimination of False positive results caused by DNA contamination provides superior accuracy – saving lives and $Billions of dollars in those industries.

ADDITIONAL INFORMATION

Weight 5 lbs
Dimensions 11.25 x 9.5 x 8 in
Volume 1 mL


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Packaging size: 20 to 40 Reactions per mL
Storage temperature: -20°C
Materials supplied:
  • One vial (1.5 ml) of RNAase and DNAase free water
  • One vial (1.5 ml) of 25 mM MgCl2
Taq storage buffer: 20 mM Tris, pH 8.0
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% IGEPAL CA-630
50% glycerol

PCR mix:

Component volume  Final concentration
2X Master Mix 10 µl 1X
3 µM Forward Primer 2 µl  0.3 µM
3 µM Reverse Primer 2 µl 0.3 µM
Template DNA  Varies < 20 ng/µl
Water Adjust for 20 µl final volume



PCR cycling set-up:

Initial Denaturation 95°C for 2 min
Cycling (25-35 cycles)
Denaturation 95°C for 30 sec
Annealing 0-68°C for 30 sec
Extension 72°C for 1 min/kb
Final Extension 72°C for 5 min
Hold 4°C indefinitely

Notes:

DNA templates should preferably be pure. Chemical agents, ionic detergents (such as SDS) and other DNA damaging agents should be avoided. For routine applications, 25 to 35 PCR cycles is usually enough to provide sufficient copies of the target sequence.

Usage:

This product is for research use only in molecular biology applications and is not intended for use in diagnostic procedures. Purchase of this product does not include a license to perform any patented application; therefore it is the users responsibility to determine if any license agreement might be needed prior usage of this product. Practicing real-time require additional licensing from Roche or Applied Biosystems.

References:

  1. Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. (1990). PCR Protocols: A
    Guide to Methods and Applications. Academic Press, New York.
  2. Kellogg, D. E., Rybalkin, I., Chen, S., Mukhamedova, N., Vlasik, T. et al. (1994)
    Biotechniques 16(6):1134-7.
  3. Mullis, K. B. (1991) PCR Methods Appl 1(1):1-4.

  4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  5. D’Aquila, R. T., Bechtel, L. J., Videler, J. A., Eron, J. J., Gorczyca, P. et al. (1991)
    Nucleic Acids Res 19(13):3749.
  6. Chou, Q., Russell, M., Birch, D. E., Raymond, J. and Bloch, W. (1992) Nucleic Acids
    Res 20(7):1717-23.
  7. Erlich, H. A., Gelfand, D. and Sninsky, J. J. (1991) Science 252(5013):1643-51.
    Guide to Methods and Applications. Academic Press, New York.