SAMURAI TAQ DNA POLYMERASE

Highly Purified for Academia & Research


A highly purified, low DNA Taq polymerase, Samurai Taq is designed for a wide range of gene amplification applications. Intended for academic and research use, the Taq polymerase is priced affordably for students, teachers, and researchers.

FEATURES:

  1. Low DNA background perfect for gene amplification
  2. Best Research Value – highly purified DNA Taq polymerase – minimal DNA

 

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PRODUCT DESCRIPTION

Pharmozyme’s Samurai Taq DNA polymerase is from Thermus aquaticus. It is a thermostable enzyme with 5’—–3’ DNA polymerase activity and 5′—–3’ exonuclease activity, while lacking 3’—–5’ exonuclease (proofreading) activity.

Applications

  • DNA amplification (PCR)
  • RT-PCR
  • DNA sequencing

ADDITIONAL INFORMATION

Weight 5 lbs
Dimensions 11.25 x 9.5 x 8 in

PCR mix:

 50 reaction volume  Final concentration

Water (ddH2O)

 adjust for 50 µl final volume
10X PCR Buffer 5 µl 1X
10 mM dNTPs 1 µl 0.2 mM of eac
10 µM Forward Primer 1.5 µl 0.3 µM
10 µM Reverse Primer 1.5 µl 0.3 µM
Template DNA Varies < 0.02 µg/µl
Taq DNA Polymerase 0.5 µl 2.5 U/50 µl

PCR cycling set-up (25-35 cycles)

Initial Denaturation 95˚C for 2 min
Denaturation 95˚C for 30 sec
Annealing 50˚- 68˚C for 30 sec
Extension 72˚C for 1 min/kb
Final Extension 72˚C for 5 min
Hold 4˚C indefinitely

PCR mix:

 50 reaction volume  Final concentration

Water (ddH2O)

 adjust for 50 µl final volume
10X PCR buffer  5 µl  1X
10 mM dNTPs  1 µl  0.2 mM of each
10 µM Forward primer  1.5 µl  0.3 µM
10 µM Reverse primer  1.5 µl  0.3 µM
Template DNA  Varies  < 0.02 µg/µl
Taq DNA polymerase  (Volume) µl  2.5 U/50 µl

PCR cycling set-up:

Initial Denaturation 95°C for 2 min
Cycling (25-35 cycles)
Denaturation 95°C for 30 sec
Annealing 0-68°C for 30 sec
Extension 72°C for 1 min/kb
Final Extension 72°C for 5 min
Hold 4°C indefinitely


Notes:

DNA templates should preferably be pure. Chemical agents, ionic detergents (such as SDS) and other DNA damaging agents should be avoided. For routine applications, 25 to 35 PCR cycles is usually enough to provide sufficient copies of the target gene.

Usage:

This product is intended for molecular biology research applications and is not for use in diagnostic procedures. Purchase of this product does not include a license to perform any patented applications; therefore it is the user responsibility to determine if any license agreement might be needed prior to usage of this product. Practicing real-time PCR may require additional licensing from Roche or Applied Biosystems.

References:

  1. “2012 Annual Report.” Life Technologies, Thermo Fisher Scientific Inc. 2013. Web.
  2. “Building Two Great Companies From One.” Agilent. Agilent, 2014. Web.
  3. “From Sample to Insight.” Qiagen Annual Report. Qiagen, 2014. Web.
  4. “Meridian Annual Report.” Meridian Bioscience. Meridian Bioscience, 2014. Web.
  5. “The Biotechnology Company.” Takara Bio Annual Report 2014. Web.
  6. Chou, Q., Russell, M., Birch, D. E., Raymond, J. and Bloch, W. (1992) Nucleic Acids Res 20(7):1717-23.
  7. D’Aquila, R. T., Bechtel, L. J., Videler, J. A., Eron, J. J., Gorczyca, P. et al. (1991) Nucleic Acids Res 19(13):3749.
  8. Erlich, H. A., Gelfand, D. and Sninsky, J. J. (1991) Science 252(5013):1643-51. Guide to Methods and Applications. Academic Press, New York.
  9. Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T.J. (1990). PCR Protocols: A Guide to Methods and Applications. Academic Press, New York.
  10. Kellogg, D. E., Rybalkin, I., Chen, S., Mukhamedova, N., Vlasik, T. et al. (1994) Biotechniques 16(6):1134-7.
  11. Mullis, K. B. (1991) PCR Methods Appl 1(1):1-4.
  12. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.